
The Jamaican, 29, lived up to his billing as the saviour of athletics as he held off the challenge of the two-time drug cheat to win in 9.79 seconds.
Pre-race favourite Gatlin had to settle for silver in Beijing.
"Coming back from injury I've had a lot of doubters, it's been tough," Bolt said after winning a ninth world title.
He added: "For me to come to the championships and defend my title is a good feeling.
"I definitely think this was my hardest race. I've been through a lot this season."
Bolt, back at the scene of his first triple Olympic triumph in 2008, called his Munich-based doctor, Hans-Wilhelm Muller-Wohlfahrt, "a lifesaver".
He also insisted he did not feel the pressure to win for his sport, only to continue his own global domination.
And that looked on course to end after the semi-finals when Gatlin clocked 9.77 seconds, while Bolt almost tripped out of the blocks and had to fight all the way to the line to snatch the win.
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